用途:生化研究。为催化己糖6位上由ATP使之产生磷酸化反应的酶。对α-D葡萄糖的亲和性很高(Km=1x10-5M),对其他的己糖也可以进行磷酸化。此反应的平衡向右偏移。该酶可以被Mg2+,K活化,但可被HS试剂阻抑。
别名:己糖磷酸激酶;HK;Hexokinase from Saccharomyces cerevisiae;ATP:D-Hesxose-6-phosphotransferase;Hexokinase from yeast;
分子量:54KD-110KD
CAS:9001-51-8
活力:>25 units/mg protein
最适pH:7.5-9.0
溶解度:soluble in cold water (0.5-1.0 unit/ml) or citrate buffer, pH 7.0
酶活定义:One unit will phosphorylate 1.0 μmole of D-glucose per min at pH 7.6 at 25 °C
简单说明:Catalyzes the phosphorylation of D-hexose sugars at the C6 position utilizing ATP as a phosphate source. ATP + D-glucose→ADP + D-glucose 6-phosphate. Reconstitute with water or citrate buffer, pH 7.0. Studies have shown excellent stability during repeated freezing and thawing over a period of at least 30 days. Hexokinase is a dimeric protein with two equal 55 kDa monomers
保存:-20℃
From Sigma